Short Description
The UC Enuity Advanced cryo-ultramicrotome with cryo chamber from Leica Microsystems is a key research infrastructure for specimen preparation in high-resolution light and electron microscopy. It enables the production of semi- and ultrathin sections as well as smooth surfaces for LM, TEM, SEM, and AFM under both room-temperature and cryogenic conditions. A motorized segment arc and knife block with automated setup functions provide precise, reproducible, and efficient adjustment of cutting parameters. An intuitive, configurable user interface supports standardized workflows and reduces user-related errors. The integrated cryo chamber allows processing of temperature-sensitive and hydrated biological specimens, for example after high-pressure freezing or plunge freezing, thereby helping to preserve native ultrastructure. In combination with a stereomicroscope for sample alignment and optional components such as an antistatic device, the system ensures stable section quality even for challenging samples (e.g. resins, plastics, tissues prone to cutting artefacts).
The instrument is suitable for research in cell and tissue biology, neurobiology, materials and polymer science, as well as for correlative light and electron microscopy. Owing to its modular upgradability and the possibility to establish standardized protocols, the cryo-ultramicrotome serves as a sustainable infrastructure resource for collaborative projects, methodological development, and training in ultrastructural analysis.
Contact Person
Dr. rer. nat. Arndt Rohwedder
Research Services
For details please contact the responsible person.
Methods & Expertise for Research Infrastructure
The “Cryo-Ultramicrotome UC Enuity Advanced” provides highly precise preparation of semi- and ultrathin sections as well as smooth surfaces from resin-embedded and cryo-preserved specimens for light and electron microscopy. Combined with the existing expertise at the Center for Medical Research, the methodological portfolio includes conventional ultramicrotomy for TEM and SEM ultrathin sections, cryo-ultramicrotomy of sensitive, hydrated biological samples (e.g. cells, tissues, organoids), and surface preparation for correlative light and electron microscopy and AFM.
The facility staff have long-standing experience in specimen preparation for ultrastructural analysis, including optimization of embedding protocols, reduction of preparation artefacts, and the establishment of standardized workflows for reproducibility and quality assurance. This enables efficient support of external users in project planning, in choosing appropriate fixation and embedding strategies, and in training in ultramicrotomy techniques. The infrastructure is suitable for collaborative projects in cell and tissue biology, pathology, neurobiology, materials and biomaterials research and provides a solid basis for methodological development and training activities.