Short Description
NGS corefacility.
The Facility assists with Sample handling, library preparations and Illumina sequencing for various high-throughput sequencing applications, including transcriptome analysis (RNA, small RNA, miRNA -seq), single-cell multiomics (RNA, V(D)J, ATAC, etc.), and spatial transcriptomics (Visium and Xenium in situ).
Please refer to our homepage for further info. https://ngsfacility.i-med.ac.at
Contact Person
Anne Krogsdam
Research Services
NGS project consultations, QC (Qubit and bioanalyzer), Library preparations, Sequencing, data delivery.
Methods & Expertise for Research Infrastructure
NGS library preparation and sequencing. Particularly expertise in RNA-seq / transcriptomes.
Equipment
Interaction with the Sequencing facility is open for all, either on a collaborative basis or purely as pay-for-service.
Comprehensive characterization of the prostate tumor microenvironment identifies CXCR4/CXCL12 crosstalk as a novel antiangiogenic therapeutic target in prostate cancer.
Heidegger I, Fotakis G, Offermann A, Goveia J, Daum S, Salcher S, Noureen A, Timmer-Bosscha H, Schäfer G, Walenkamp A, Perner S, Beatovic A, Moisse M, Plattner C, Krogsdam A, Haybaeck J, Sopper S, Thaler S, Keller MA, Klocker H, Trajanoski Z, Wolf D, Pircher A.
Mol Cancer. 2022 Jun 18;21(1):132.
Smooth Muscle Specific Ablation of CXCL12 in Mice Downregulates CXCR7 Associated with Defective Coronary Arteries and Cardiac Hypertrophy.
Ghadge SK, Messner M, Seiringer H, Maurer T, Staggl S, Zeller T, Müller C, Börnigen D, Weninger WJ, Geyer SH, Sopper S, Krogsdam A, Pölzl G, Bauer A, Zaruba MM.
Int J Mol Sci. 2021 May 31;22(11):5908. doi: 10.3390/ijms22115908.
PMID: 34072818
Nuclear receptor NR2F6 inhibition potentiates responses to PD-L1/PD-1 cancer immune checkpoint blockade.
Klepsch V, Hermann-Kleiter N, Do-Dinh P, Jakic B, Offermann A, Efremova M, Sopper S, Rieder D, Krogsdam A, Gamerith G, Perner S, Tzankov A, Trajanoski Z, Wolf D, Baier G.
Nat Commun. 2018 Apr 18;9(1):1538. doi: 10.1038/s41467-018-04004-2.
Targeting immune checkpoints potentiates immunoediting and changes the dynamics of tumor evolution.
Efremova M, Rieder D, Klepsch V, Charoentong P, Finotello F, Hackl H, Hermann-Kleiter N, Löwer M, Baier G, Krogsdam A, Trajanoski Z.
Nat Commun. 2018 Jan 2;9(1):32. doi: 10.1038/s41467-017-02424-0.
Impact of the Chromatin Remodeling Factor CHD1 on Gut Microbiome Composition of Drosophila melanogaster.
Sebald J, Willi M, Schoberleitner I, Krogsdam A, Orth-Höller D, Trajanoski Z, Lusser A.
PLoS One. 2016 Apr 19;11(4):e0153476. doi: 10.1371/journal.pone.0153476. eCollection 2016.
Changes in the miRNA-mRNA Regulatory Network Precede Motor Symptoms in a Mouse Model of Multiple System Atrophy: Clinical Implications.
Schafferer S, Khurana R, Refolo V, Venezia S, Sturm E, Piatti P, Hechenberger C, Hackl H, Kessler R, Willi M, Gstir R, Krogsdam A, Lusser A, Poewe W, Wenning GK, Hüttenhofer A, Stefanova N.
PLoS One. 2016 Mar 10;11(3):e0150705. doi: 10.1371/journal.pone.0150705. eCollection 2016.
Heidegger I, Fotakis G, Offermann A, Goveia J, Daum S, Salcher S, Noureen A, Timmer-Bosscha H, Schäfer G, Walenkamp A, Perner S, Beatovic A, Moisse M, Plattner C, Krogsdam A, Haybaeck J, Sopper S, Thaler S, Keller MA, Klocker H, Trajanoski Z, Wolf D, Pircher A.
Mol Cancer. 2022 Jun 18;21(1):132.
Smooth Muscle Specific Ablation of CXCL12 in Mice Downregulates CXCR7 Associated with Defective Coronary Arteries and Cardiac Hypertrophy.
Ghadge SK, Messner M, Seiringer H, Maurer T, Staggl S, Zeller T, Müller C, Börnigen D, Weninger WJ, Geyer SH, Sopper S, Krogsdam A, Pölzl G, Bauer A, Zaruba MM.
Int J Mol Sci. 2021 May 31;22(11):5908. doi: 10.3390/ijms22115908.
PMID: 34072818
Nuclear receptor NR2F6 inhibition potentiates responses to PD-L1/PD-1 cancer immune checkpoint blockade.
Klepsch V, Hermann-Kleiter N, Do-Dinh P, Jakic B, Offermann A, Efremova M, Sopper S, Rieder D, Krogsdam A, Gamerith G, Perner S, Tzankov A, Trajanoski Z, Wolf D, Baier G.
Nat Commun. 2018 Apr 18;9(1):1538. doi: 10.1038/s41467-018-04004-2.
Targeting immune checkpoints potentiates immunoediting and changes the dynamics of tumor evolution.
Efremova M, Rieder D, Klepsch V, Charoentong P, Finotello F, Hackl H, Hermann-Kleiter N, Löwer M, Baier G, Krogsdam A, Trajanoski Z.
Nat Commun. 2018 Jan 2;9(1):32. doi: 10.1038/s41467-017-02424-0.
Impact of the Chromatin Remodeling Factor CHD1 on Gut Microbiome Composition of Drosophila melanogaster.
Sebald J, Willi M, Schoberleitner I, Krogsdam A, Orth-Höller D, Trajanoski Z, Lusser A.
PLoS One. 2016 Apr 19;11(4):e0153476. doi: 10.1371/journal.pone.0153476. eCollection 2016.
Changes in the miRNA-mRNA Regulatory Network Precede Motor Symptoms in a Mouse Model of Multiple System Atrophy: Clinical Implications.
Schafferer S, Khurana R, Refolo V, Venezia S, Sturm E, Piatti P, Hechenberger C, Hackl H, Kessler R, Willi M, Gstir R, Krogsdam A, Lusser A, Poewe W, Wenning GK, Hüttenhofer A, Stefanova N.
PLoS One. 2016 Mar 10;11(3):e0150705. doi: 10.1371/journal.pone.0150705. eCollection 2016.