Short Description
The CytoFLEX-S System (B2-R2-V2-Y3, Beckman-Coulter) is a highly sensitivity flow cytometer/cell analyzer designed for multi-parametric analysis of microbial populations, single-cell characterization, and small particle detection. It is equipped with a 96-well plate loader allowing for automated analysis.
Contact Person
Dr. Marc Mußmann
Research Services
Expertise in sample fixation, cell handling and staining as preparation for flow cytometric analysis, along with guidance on fluorochrome selection and measurement parameters.
Methods & Expertise for Research Infrastructure
The CytoFLEX S System (B2-R2-V2-Y3) is a highly sensitivity flow cytometer designed for multi-parametric analysis of microbial populations, single-cell characterization, and small particle detection.
It is equipped with four excitation lasers:
1. Violet (405 nm),
2. Blue (488 nm)
3. Yellow-Green (561 nm)
4. Red (638 nm),
and 9 fluorescence detection channels.
The system also includes Violet Side Scatter (VSSC), which enhances small particle detection (<200 nm), allowing for the analysis of viruses, bacteriophages, extracellular vesicles (EVs), and other sub-micron particles.
Primary Applications in our lab is microbial cell and bacteriophage counting using fluorescent dyes such as SYBR® Green I.
Mounted to the Cytoflex-S is a 96-deep well plate-loader that allows for automatic sampling and analysis. Single tube (5 ml) measurements are also possible.
A mandatory hands-on training session (~2 hours) is required before independent use of the device. Ongoing support is available throughout experiments as needed.