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Large equipment

STED-Minflux Microscope

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Institute of Science and Technology Austria (ISTA)

Klosterneuburg | Website

Open for Collaboration

Short Description

The Abberior Miriava Polyscope comes with a multitude of imaging modalities that allow imaging beyond the diffraction limit of light and approach molecular resolution: Confocal (200 nm) > Matrix detector (70 nm) > 2D / 3D STED (20 nm) > Minflux (3.2 nm). Additional advanced technologies to correct for aberrations. The system is equipped with a incubation chamber, stage-heater an options for humidity – CO2 and O2 control.

Contact Person

Gabriel Krens

Research Services

Imaging Modes:

Confocal: up to 200nm resolution
Matrix: Array detector that allows up to 70nm resolution
STED : 2 color STED in 2D (20nm) or 3D (< 100nm), with
Minflux: nanometer-scale resolution (3.2 nm)

Imaging Applications:

Adaptive Optics (Rayshape): Deformable mirror-based correction of spherical aberrations for all beams
Adaptive illumination for STED (Flexposure): Light dose minimization to leverage live cell imaging and minimize photo-bleaching
Life Time Imaging (Timebow): Life time measurement and Life Time filtered STED
Spectral detection (400-800nm; Matrix)
Deconvolution using the Truesharp module

Methods & Expertise for Research Infrastructure

Microscopy with different imaging modalities, data analysis, basic training, sample preparation consulting

Allocation to research infrastructure

Imaging Facility

Terms of Use

Terms of use will be defined in a scientific collaboration agreement.

Contact

Gabriel Krens
Imaging & Optics Facility
+43 2243 9000 1043
IOF@ist.ac.at
https://ist.ac.at/en/research/scientific-service-units/imaging-optics-facility/

Location

Location on map

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