Operetta CLS System

University of Veterinary Medicine Vienna

Vienna | Website

Open for Collaboration

Short Description

The PerkinElmer Operetta CLS is an innovative high-content/high-throughput analysis system with a confocal option. The device is specialized in obtaining more physiologically relevant information through complex image analyses at high throughput.

The unique features of the PerkinElmer Operetta CLS™ are diverse and include, among other things, an excitation spectrum equipped with 8 LEDs covering a range from UV to near-infrared, a 4-position emission filter wheel, and three water-immersion objectives (20x, NA 1.0; 40x, NA 1.1; 63x, NA 1.15), which enable higher Z-resolution for 3D analyses as well as shorter measurement times.

Furthermore, the system is controlled by Harmony, a user-friendly software platform, and plate data can be automatically transferred to the PerkinElmer Columbus image storage and analysis system.

Contact Person

Doris Wilflingseder

Research Services

Automated imaging of large numbers of sample; quantitative analysis of cellular phenotypes from fluorescence, brightfield, or phase-contrast images; suitable for siRNA/CRISPR screens, or drug discovery studies; assistance in confocal / widefield / brightfield / phase-contrast imaging; assistance in multi-color fluorescence imaging

Methods & Expertise for Research Infrastructure

Using the PerkinElmer Operetta CLS, we perform high-content, high-throughput imaging and quantitative analysis of complex 3D tissues and organoids. The system’s confocal capabilities and water-immersion objectives enable high-resolution 3D reconstruction and detailed phenotypic characterization. We conduct automated test series and compound screenings in multiwell formats, as well as functional live-cell analyses, including mucociliary clearance assays and dynamic cellular response measurements under physiologically relevant conditions.