Short Description
The TissueFAXs is an automated workstation on a Zeiss observer microscope for fluorescence and transmission light. It is equipped with a 12 slides or 2 plate stage, 5 fluorescence channels and objectives of choice. Preview objective is on our system at the moment the 2.5x, but it is possible to use the 5x on request. For acquisition the system offers a 20x objective. It is also possible to combine fluorescence and transmission images.
The HistoQuest & TissueQuest, which is the analysis software for transmission and fluorescence is available on a workstation in the facility.
The system supports following applications (- or combinations thereof):
- Automated multi-channel image acquisition
- Individual regions of interest (ROI) definable by graphical tools
- Automated single cell detection by patented algorithms
- Suitable for cryocut and paraffin sections, cell cultures, TMA's and smears
- Forward Gating from the individual cell in the image to the dot in the scattergram
- Backward Gating from the individual dot or gate to the corresponding cells in the sample
- DotPlot operations – analysis of the image processing results via gating and additional DotPlots
- Histograms and overlays
Technical Data:
Wide Field Illumination:
LED
Fluorescence Light Source, X-Cite Series 120PC Q
Filter:
Filter Sets: DAPI, GFP, Cy3, Cy5, C7
Objectives:
2.5x/0.085 EC-Plan Neofluor
5x/ 0.16 Plan-Apochromat on request
20x/ 0.5 EC- Plan-Neofluor
40x/ 06 LD-Plan Neofluor PH2 on request
Camera:
Baumer HXG40c = transmission
High Speed Color Camera
CMOS
4 Megapixels
2048x2048 pixels
max. 56 fps
Hamamatsu Orca Flash 4.0
High Speed Fluorescence Camera
CMOS Sensor FL-400
2048x2048 pixels
100 fps
Peltier Cooling
Software:
TissueFAXs acquisition software
HistoQUEST
TissueQUEST
Fees:
28€/h introduction
5€/h running time
Contact Person
Sabine Rauscher
Research Services
Advice and support in setting up protocols
Trainings for the equipment of the facility
Assistance in using the equipment
Data analysis
Imaging Workshops
Equipment maintenance
Methods & Expertise for Research Infrastructure
The Core Facility Imaging is located in the Anna Spiegel Building – Center of Translational Research. In the area of Imaging a large variety of techniques is available to address complex problems in vitro, in situ and in vivo. The Imaging Core Facility provides state-of-the-art equipment for committed work in this field. Upon request, experienced staff members offer their support and know-how for establishing and troubleshooting of novel experimental strategies.
The available systems support high resolution imaging of tissue sections or cells, 3D and 4D acquisition, visualization of protein-protein interactions (like FRET) and live cell imaging. Furthermore the facility offers workstations to the users, which allow an onsite evaluation and processing of their data with the suitable software.
In addition the area of microscopy the core facility houses a small histology unit for sample preparation. This is equipped with one cryostat and two microtomes. The microtomes are only to cut paraffin embedded material for the purpose of RNA and DNA extraction.
For performing live cell imaging the facility provides a fully equipped cell culture lab.
To use the instruments in the facility a training given by the facility staff is an indispensable prerequisite. It is prohibited to use microscopes without this training and moreover users who have been trained are not permitted to train other users! During the training users will get the login data for our booking calendar and are then able to book the systems by themselves.
The opening hours of the facility are Mo.-Fr. between 9.00 and 17.00. It is possible to use the facility out of opening hours, however in this case please contact the facility staff early enough in order to ensure practical arrangements!
Allocation to research infrastructure
The opening hours of the facility are Mo.-Fr. between 9.00 and 17.00. It is possible to use the facility out of opening hours, however in this case please contact the facility staff early enough in order to ensure practical arrangements!
fees (TissueFAXSi PLUS): 5 €/h, introduction: 28 €/h