Kurzbeschreibung
The Oroboros is the Bioenergetics Platform for high-resolution respirometry (HRR) for mitochondria and cell research, unique for precision OXPHOS analysis at controlled oxygen levels using small amounts of biological sample.
Research areas: fundamental bioenergetics, analysis of mitochondrial and metabolic diseases, field of preventive mitochondrial medicine.
Find solutions for and study mitochondrial function in cancer, diabetes, aging, neurodegeneration, exercise physiology and mitochondrial medicine.
Research Services
OXPHOS Analysis of mitochondrial function in disease and healthy controls.
Methoden & Expertise zur Forschungsinfrastruktur
The Oroboros is the Bioenergetics Platform for high-resolution respirometry (HRR) for mitochondria and cell research, unique for precision OXPHOS analysis at controlled oxygen levels using small amounts of biological sample. The Oroboros is available as two models: Oroboros NextGen-O2k and Oroboros O2k. Both provide the basis for HRR, supported by the DatLab software, with high flexibility for add-on Modules.
Both Oroboros models provide the basis for HRR applications combined with measurement of ROS production, mitochondrial membrane potential, ATP production, Ca2+, pH, and PhotoBiology. All Oroboros Modules are supported by the Oroboros NextGen-O2k, which is the only model supporting the Redox Modules (Q-Module and NADH-Module), and the NO-Module.
Small amounts of biological samples can be used for bioenergetic and OXPHOS analysis, ranging from isolated mitochondria, permeabilized tissues and permeabilized cells, to living cells and tissues slices.
The globally tested and trusted high-resolution Oroboros prioritizes both quality and scientific research output in the field of mitochondrial physiology and pathology, extended to PhotoBiology. The Oroboros is a robust sole-source instrument with a long life time and low running costs. Expert service is provided by the Oroboros Open Support.
The modular concept is supported by the specially developed Oroboros Software, DatLab, guiding the user step-by-step through the desired experiment. In addition, international Oroboros Workshops are provided on a regular basis, allowing for hands-on practical training.
Oroboros Instruments boasts over 30 years of experience and offers the leading instrument on the market in mitochondrial physiology and pathology. The Oroboros is developed and produced in Austria by Oroboros Instruments in close cooperation with WGT-Elektronik GmbH & Co KG.
The Oroboros Network includes and connects reference laboratories worldwide and labs, hospitals, research centers, pharmaceutical and biotech companies in over 50 countries worldwide.
For further information have a look at our website: https://www.oroboros.at
- NextGen-O2k H2020 SME Phase 2
- COST Action 15203 MitoEAGLE
- Marie Sklodowska-Curie Projects TRACT and TRANSMIT
Gnaiger E (2025) Mitochondrial respiratory function in living cells. Bioenerg Commun 2025.5. https://doi.org/10.26124/bec.2025-0005
Baglivo E, Cardoso LHD, Cecatto C, Gnaiger E (2024) Stability of mitochondrial respiration medium used in high-resolution respirometry with living and permeabilized cells. MitoFit Preprints 2024.7. https://doi.org/10.26124/mitofit:2024-0007 — Published 2024-12-06 Bioenerg Commun 2024.8
Komlódi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. Bioenerg Commun 2021.3. https://doi.org/10.26124/bec:2021-0003
Gnaiger E, Aasander Frostner E, Abdul Karim N, Abdel-Rahman EA, Abumrad NA, Acuna-Castroviejo D, Adiele RC, et al (2019) Mitochondrial respiratory states and rates. MitoFit Preprint Arch doi:10.26124/mitofit:190001.v6.
Gnaiger E (2019) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Mitochondr Physiol Network 24.05. Oroboros MiPNet Publications, Innsbruck:112 pp.
Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38.
Further publications are accessible online:
https://wiki.oroboros.at/index.php/O2k-Publications:_Topics