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Large equipment

BD FACSAria™ Fusion

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JKU - Johannes Kepler University Linz

Linz | Website

Open for Collaboration

Short Description

The BD FACSAria™ Fusion is a high-speed cell sorter and is integrated into a Class II safety cabinet (European Standard 12469) for operator and product protection. With its five lasers (355nm, 405nm, 488nm, 561nm, 640nm) and 18 fluorescence channels, it enables complex high-speed cell sorting and high-purity sorting of up to four subpopulations in different formats as well as single-cell sorting in defined plate formats.

Laser and fluorescence detection channels:

Ultraviolet Laser (355nm): A: 740/35 BP, 690 LP, B: 450/50 BP, 410 LP, C: 379/28 BP

Violet Laser (405nm): A: 780/60 BP, 750 LP, B: 710/50 BP, 690 LP, C: 660/20 BP, 630 LP, D: 610/20 BP, 595 LP, E: 525/50 BP, 505 LP F: 450/50 BP

Blue Laser (488nm): A: 695/40 BP, 655 LP, B: 530/30 BP, 502 LP

Yellow-Green Laser (561nm): A: 780/60 BP, 735 LP, B: 670/14 BP, 630 LP, C: 610/20 BP, 600 LP, D: 582/15 BP

Red Laser (640nm): A: 780/60 BP, 755 LP, B: 730/45 BP, 690 LP, C: 670/30 BP

Contact Person

Dr. Anna-Maria Lipp

Research Services

Expert advice on planning and preparing experiments and carrying out cell sorting analyses.

Methods & Expertise for Research Infrastructure

- Multicolor analysis of up to 18 fluorescence and two scattering options simultaneously.
- 4 different nozzle sizes (70µm, 85µm, 100µm and 130μm)
- Simultaneous sorting of up to 4 subpopulations
- 4 different standard sorting modes available (purity, yield, single cell, 4-way purity)
- Index sorting when sorting individual cells
- Sorting into multiwell plates

Allocation to research infrastructure

Core Facility Cytometry

Terms of Use

After contacting and consulting the experimental conditions, the device is operated exclusively by the Core Facility.

Contact

Leitung Core Facility
Dr. Anna-Maria Lipp
Zentrum für Medizinische Forschung - Core Facility Zytometrie
+43 664 9601459
anna-maria.lipp@jku.at
https://www.jku.at/medizinische-fakultaet/forschung/zentrum-fuer-medizinische-forschung/core-facilities/zytometrie/

Location

Location on map

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