University of Vienna
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Short Description
The Thermo Exploris 480 is a state-of-the-art high resolution massspectrometer. The instrument is coupled to high-performance liquid chromatography (HPLC) and is used for metabolomics and exposomics experiments within the ERC project EXPOMET.
The instrument is a hybrid quadrupol-orbiotrap mass spectrometer and can be operated with a maximum mass resolution of 480000 (FWHM at m/z 200). The instrument can maximize the coverage of fragment spectra using the AcquireX workflow. Furthermore, multistage fragmentation can be used to assist structural elucidation of unknown substances. The high mass resolving power combined with the excellent sensitivity of the instrument allows the parallel identification of new substances and quantification of analytes at trace levels, which is a prerequisite for human biomonitoring (HBM) and exposomics analysis. The improved ESI source and ion transfer optics allow robust measurements for large sample cohorts.
The instrument is coupled to a Vanquish Duo HPLC and can be used for high-throughput applications. Two different HPLC methods (e.g., HILIC and reversed phase) can be used in parallel or alternating to maximize sample throughput and to generate metabolomics and exposomics datasets in a single workflow.
Contact Person
Univ.-Prof. Dipl.-Ing. Dr. Benedikt Warth
Research Services
No specific research services are currently offered for this instrument. If you are interested in cooperation, please reach out directly to Professor Benedikt Warth (benedikt.warth@univie.ac.at).
Methods & Expertise for Research Infrastructure
Non-targeted metabolomics, exposomics, and suspect screening
Allocation to research infrastructure
https://cordis.europa.eu/project/id/101043321
Nature Communications 2022: Next-generation biomonitoring of the early-life chemical exposome in neonatal and infant development (https://www.nature.com/articles/s41467-022-30204-y)
Environment International 2022: Elucidation of xenoestrogen metabolism by non-targeted, stable isotope-assisted mass spectrometry in breast cancer cells (https://www.sciencedirect.com/science/article/pii/S0160412021005651)