Andromeda X Package

University of Veterinary Medicine Vienna

Wien | Website

Open for Collaboration

Short Description

The NanoTemper Andromeda X is a state-of-the-art biophysical instrument designed to measure the stability of RNAs and optimize the expression and quality of recombinant proteins in the early stages of drug discovery. It uses Nano Differential Scanning Fluorimetry (nanoDSF), a technique for monitoring protein stability by measuring changes in intrinsic fluorescence as a function of temperature — it has recently been shown that the same principle can also be used for position-specific stability measurements of RNAs. For proteins, the principle is based on the fact that the environment of the tryptophan and tyrosine residues in a protein changes during thermal denaturation, leading to a shift in the emission spectrum. For RNAs, specific fluorescent markers must be incorporated at specific sites. During heating, the protein unfolds, and the exposure of the hydrophobic regions leads to a change in fluorescence intensity, which is detected in real time. In RNAs, unfolding causes a change in the environment of the incorporated fluorophore. By analyzing the temperature at which these fluorescence changes occur, nanoDSF provides information about the melting temperature (Tm) of the protein/RNA, which reflects its stability.

Contact Person

Sebastian Glatt

Research Services

RNA stability, RNA folding, binding of chemical molecules to RNA

Methods & Expertise for Research Infrastructure

It uses Nano Differential Scanning Fluorimetry (nanoDSF), a technique for monitoring protein stability by measuring changes in intrinsic fluorescence as a function of temperature—it has recently been shown that the same principle can also be used for position-specific stability measurements of RNAs.