Monolith Pico Red

University of Veterinary Medicine Vienna

Wien | Website

Open for Collaboration

Short Description

The NanoTemper Monolith Pico Red is a MicroScale Thermophoresis (MST)-based instrument designed to quantify biomolecular interactions in solution under near-native conditions. It enables the rapid determination of binding affinities across a broad dynamic range of picomolar to millimolar concentrations. MST is a technique for measuring the movement of molecules in a temperature gradient, known as thermophoresis, which depends on the magnitude of the charge and the hydration shell of the molecules. When a molecule is exposed to a temperature gradient, it moves toward cooler or warmer regions depending on its properties, and this movement can be detected by observing the change in fluorescence of a labeled molecule.

In MST, a fluorescently labeled target molecule is exposed to a temperature gradient, and the change in its migration pattern is tracked in real time. When a ligand binds to the target molecule, it alters its thermophoretic behavior, leading to a measurable shift in the fluorescence signal. This enables the determination of binding affinity, stoichiometry, and the kinetics of molecular interactions without the need for complex labeling or surface immobilization.

Contact Person

Sebastian Glatt

Research Services

MicroScale Thermophoresis (MST), RNA and Protein Interaction Assays

Methods & Expertise for Research Infrastructure

MST is a technique for measuring the movement of molecules in a temperature gradient, known as thermophoresis, which depends on the magnitude of the charge and the hydration shell of the molecules.