Short Description
The QX200 AutoDG Droplet Digital PCR System simplifies the Droplet Digital PCR (ddPCR™) workflow, reducing hands-on time and eliminating user-to-user variability. The AutoDG Instrument brings scalability and flexibility to Bio-Rad’s ddPCR technology, the most precise and sensitive digital PCR solution for the absolute quantification of target DNA molecules.
AutoDG Instrument:
Guides setup and loading with large, color touch-screen interface and LED lighting
Compatible with both the QX100™ and QX200™ Droplet Digital™ PCR Systems
Generates droplets for 96 ddPCR reactions in under 45 minutes
One AutoDG Instrument can supply 4–5 droplet readers continuously
HEPA-filtered enclosure reduces contamination and allows the system to be placed in any lab environment
User-definable plate layout with increments of 8 wells provides run-to-run flexibility
Droplet Digital PCR Technology:
Flexible digital PCR chemistry — optimized for TaqMan Hydrolysis Probe and EvaGreen Assays
Droplet partitioning by the QX200 Droplet Digital PCR system reduces bias from amplification efficiency and PCR inhibitors
Convenient assay design — standard curves are not required
Contact Person
Univ.-Prof. Mag. Dr. Michael Traugott
Research Services
Momentan keine Research Services für dieses Gerät.
Die Forschungsinfrastruktur ist "Open for Collaboration". Kommerzielle Kooperationen sind nicht möglich.
Methods & Expertise for Research Infrastructure
Expertise bzw. Einsatz des Systems besteht im Bereich der Quantifizierung von DNA in Umweltproben (z.B. Wasser, Boden, Nahrungsproben), insbesondere wenn DNA-Konzentrationen sehr gering sind.
Nutzungsbedingungen werden im Rahmen einer wissenschaftlichen Kooperation definiert. Keine kommerzielle Nutzung möglich. Bei Interesse an einer Kooperation oder Zusammenarbeit bitten wir Sie um Kontaktaufnahme.
- FFG Bridge-1 Projekt "eDNA-AlpFisch" (Traugott)
- FFG Bridge-1 Projekt "VectorDetect" (Traugott)
- FWF Projekt "Assessing food web dynamics to improve biocontrol of pests" (Traugott)
Limnologie
- FWF-Projekt "Chemolithoautotrophe Kohlenstoffdioxid-Fixierung in Seen" (Alfreider)
- FWF-Projekt "Mechanismen und Signifikanz von Genomgrößenvariation bei Rotatorien" (Stelzer)
Mikrobiologie (u.a.)
- Euregio IPN 94-B29 (Präg)
- FWF-Projekt „Luftdruck-Effekte auf höher wandernde alpine Ökosysteme“ (I 5241, Illmer)
Alfreider A, Tartarotti B. Spatiotemporal dynamics of different CO2 fixation strategies used by prokaryotes in a dimictic lake. Sci Rep. 2019; 9:15068. doi: 10.1038/s41598-019-51584-0.
Harringer M, Alfreider A. Primer evaluation and development of a droplet digital PCR protocol targeting amoA genes for the quantification of Comammox in lakes. Sci Rep. 2021; 11:2982. doi: 10.1038/s41598-021-82613-6.
Praeg, Nadine; Schachner, Iris; Schuster, Lisa; Illmer, Paul (2021): Carbon-dependent growth, community structure, and methane oxidation performance of a soil-derived methanotrophic mixed culture.
In: FEMS Microbiology Letters 368/2, No. fnaa212. (DOI) (Weblink)
Praeg, Nadine; Illmer, Paul (2020): Microbial community composition in the rhizosphere of Larix decidua under different light regimes with additional focus on methane cycling microorganisms.
In: Scientific Reports 10, No. 22324. (Volltext) (DOI) (Weblink)
Thalinger B., Pütz Y. & Traugott M. (2021): Endpoint PCR coupled with capillary electrophoresis (celPCR) provides sensitive and quantitative measures of environmental DNA in singleplex and multiplex reactions. PlosOne, 16(7): e0254356, https://doi.org/10.1371/journal.pone.0254356
Thalinger B., Rieder A., Teuffenbach A., Pütz Y., Schwerte T., Wanzenböck J. & Traugott M. (2021): The effect of activity, energy use, and species identity on environmental DNA shedding of freshwater fish. Frontiers in Ecology and Evolution, doi: 10.3389/fevo.2021.623718