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Core facility (CF)

Lipid Mass Spectrometry

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University of Graz

Graz | Website

Open for Collaboration

Short Description

Infrastructure (excerpt):
- Ion mobility quadrupole time-of-flight mass spectrometer (model 6560, Agilent) & 1290 UHPL and 1260 SFC II (Agilent)
- Triple quadrupole mass spectrometer (model 6470, Agilent) with UHPLC (model 1290, Agilent)
- Triple quadrupole mass spectrometer (model 6475, Agilent) with UHPLC (model 1290, Agilent)
- Triple quadrupole mass spectrometer (model 7013, Agilent) with GC (model 8890, Agilent)
- Alliance HPLC with UV and fluorescence detector (Waters)
- HP1100 HPLC with UV, fluorescence, and light scattering detector (Agilent)
- Trace GC with FID detector (Thermo)
- Various evaluation software (e.g., MassHunter, LipidData Analyzer)
- Personnel: 2 staff scientists, partially project-funded personnel, including for method

Contact Person

Dr. Gerald Rechberger

Research Services

Collaborations within the framework of basic research projects

Methods & Expertise for Research Infrastructure

Mass spectrometry with a focus on lipid analysis

Equipment

  • 6560 Ion Mobility Quadrupole-Time of Flight Mass spectrometer & 1290 UHPL und 1260 SFC II
  • 6470 LC- Triple-Quadrupole MS-System & 1290 UHPLC

Terms of Use

Scientific cooperation by arrangement. Please contact Dr. Gerald Rechberger or Dr. Thomas Züllig.

Cooperation Partners

Medical University of Graz and Graz University of Technology as part of BioTechMed Graz
Medical Universities of Graz and Vienna as part of MetAGE - Cluster of Excellence
Graz University of Technology as part of NAWI Graz
Austrian and various European universities as part of individual and EU projects

Reference Projects

MetAGE - Cluster of Excellence
https://www.metage.at/en/

OMICS Center Graz
https://omicscentergraz.at

SFB Lipotox
http://lipotox.uni-graz.at

Reference Publications

YongTian Liang, Anja Krivograd, Sebastian J Hofer, Laxmikanth Kollipara, Thomas Züllig, Sickmann Albert, Eisenberg Tobias, Stephan J Sigrist; Spermidine supplementation and protein restriction protect from organismal and brain aging independently; in: Aging (Albany NY) 2025 Jun 7;17(6):1429-1451. DOI: 10.18632/aging.206267

Schwarz, Andreas N.; Züllig, Thomas; Schicher, Maximilian; Wagner, Franz S.; Rechberger, Gerald N.: Securing food authenticity by translating triacylglycerol profiles of edible oils into a versatile identification method for pumpkin seed oil adulteration, in: Food Chemistry 463,4 (2025), 141467. DOI: 10.1016/j.foodchem.2024.141467

Tehlivets, Oksana; Almer, Gunter; Brunner, Markus S; Lechleitner, Margarete; Sommer, Gerhard; Kolb, Dagmar; Leitinger, Gerd; Diwoky, Clemens; Wolinski, Heimo; Habisch, Hansjörg; Opriessnig, Peter; Bogoni, Francesca; Pernitsch, Dominique; Kavertseva, Maria; Bourgeois, Benjamin; Kukilo, Jelena; Tehlivets, Yuriy G; Schwarz, Andreas N; Züllig, Thomas; Bubalo, Vladimir; Schauer, Silvia; Groselj-Strele, Andrea; Hoefler, Gerald; Rechberger, Gerald N; Herrmann, Markus; Eller, Kathrin; Rosenkranz, Alexander R; Madl, Tobias; Frank, Saša; Holzapfel, Gerhard A; Kratky, Dagmar; Mangge, Harald; Hörl, Gerd; : Homocysteine contributes to atherogenic transformation of the aorta in rabbits in the absence of hypercholesterolemia , in: Biomedicine & Pharmacotherapy 178 (2024), 117244. DOI: 10.1016/j.biopha.2024.117244

Cavallari, Nicola; Johnson, Johnson; Johnson, Alexander; Nagl, Christoph; Seiser, Saskia; Rechberger, Gerald N.; Züllig, Thomas; Kufer, Thomas A.; Elbe-Bürger, Adelheid; Geiselhart, Sabine; Hoffmann-Sommergruber, Karin; : Non-specific lipid-transfer proteins trigger TLR2 and NOD2 signaling and undergo ligand-dependent endocytosis in epithelial cells, in: Journal of Allergy and Clinical Immunology 154,5 (2024), 1289-1299. DOI: 10.1016/j.jaci.2024.07.015

Chalhoub, Gabriel; Jamnik, Alina; Pajed, Laura; Kolleritsch, Stephanie; Hois, Victoria; Bagaric, Antonia; Prem, Dominik; Tilp, Anna; Kolb, Dagmar; Wolinski, Heimo; Taschler, Ulrike; Zuellig, Thomas; Rechberger, Gerald N.; Fuchs, Claudia; Trauner, Michael; Schoiswohl, Gabriele; Haemmerle, Guenter: Carboxylesterase 2a deletion provokes hepatic steatosis and insulin resistance in mice involving impaired diacylglycerol and lysophosphatidylcholine catabolism, in: Molecular Metabolism 72 (2023), -. DOI: 10.1016/j.molmet.2023.101725

Gernot F Grabner, Nikolaus Guttenberger, Nicole Mayer, Anna K Migglautsch-Sulzer, Christian Lembacher-Fadum, Nermeen Fawzy, Dominik Bulfon, Peter Hofer, Thomas Züllig, Lennart Hartig, Natalia Kulminskaya, Gabriel Chalhoub, Margarita Schratter, Franz P W Radner, Karina Preiss-Landl, Sarah Masser, Achim Lass, Rudolf Zechner, Karl Gruber, Monika Oberer, Rolf Breinbauer, Robert Zimmermann; Small-Molecule Inhibitors Targeting Lipolysis in Human Adipocytes. in: J Am Chem Soc. 2022 Apr 13;144(14):6237-6250. DOI: 10.1021/jacs.1c10836

Vigor Claire, Züllig Thomas, Eichmann Thomas O., Oger Camille, Zhou Bingqing, Rechberger Gerald N., Hilsberg Lucas, Trötzmüller Martin, Pellegrino Roberto M, Alabed Husam BR, Hartler Jürgen, Wolinski Heimo, Galano Jean-Marie, Durand Thierry, Spener Friedrich: α-Linolenic acid and product octadecanoids in Styrian pumpkin seeds and oils: How processing impacts lipidomes of fatty acid, triacylglycerol and oxylipin molecular structures, in: Food Chemistry 371 (2022), 131194. DOI: https://doi.org/10.1016/j.foodchem.2021.131194

Hartler, Jürgen; Triebl, Alexander; Ziegl Andreas; Trötzmüller, Martin; Rechberger, Gerald N.; Zeleznik, Oana A.; Zierler,Kathrin A.; Torta, Federico; Cazenave-Gassiot, Amaury; Wenk, Markus R.; Fauland, Alexander; Wheelock, Craig E.; Armando, Aaron M.; Quehenberger, Oswald; Zhang, Qifeng; Wakelam, Michael J.O.; Haemmerle, Günter; Spener, Friedrich; Köfeler, Harald C.; Thallinger, Gerhard G.: Deciphering lipid structures based on platform-independent decision rules. in: Nature Methods Epub ahead of print (2017), nmeth.4470. DOI: 10.1038/nmeth.4470

Knittelfelder, Oskar; Weberhofer, Bernd; Eichmann, Thomas; Kohlwein, Sepp-Dieter; Rechberger, Gerald: A versatile ultra-high performance LC-MS method for lipid profiling, in: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences 951-952 (2014), 119 - 128. DOI: 10.1016/j.jchromb.2014.01.011.

Contact

Dr. Gerald Rechberger
Institut für Molekulare Biowissenschaften
+43-316-380 1933
gerald.rechberger@uni-graz.at
https://molekularbiologie.uni-graz.at/de/schluesseltechnologien/lipid-massenspektrometrie/

Dr. Thomas Züllig
Institut für Molekulare Biowissenschaften
+43-316-380 3954
thomas.zuellig@uni-graz.at
https://molekularbiologie.uni-graz.at/de/schluesseltechnologien/lipid-massenspektrometrie/

Location

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